![]() Statistics shown are for the comparison of triple positive cells in each condition. The combination SAPG gave 35% (± 1.8) reprogramming efficiency and 78% (± 1.9) of Atoh1-nGFP+ cells were triple positive. Reporter activation and immunostaining for anti-MyosinVIIa and anti-Parvalbumin was used to quantify triple positive cells ( Atoh1-nGFP+/MyosinVIIa+/Parvalbumin+). Reprogramming efficiency was calculated as the number of Atoh1-nGFP positive cells divided by the 5000 MEFs plated per well. ( D) All quantification was performed at 14 dpi. Atoh1-nGFP reporter activation (green) and immunostaining for anti-MyosinVIIa (red) and anti-Parvalbumin (cyan). ( C) Images of MEFs reprogrammed with Six1, Atoh1, Pou4f3, and Gfi1 (SAPG) fixed at 14 days post infection (dpi). MEFs were plated at a density of 5000 cells per well of a 96 well plate, infected with retroviral transcription factors and allowed to reprogram for 14 days prior to analysis. Mouse embryonic fibroblasts (MEFs) were isolated from Atoh1-nGFP transgenic reporter mice. ( B) Schematic of experimental design for transcription factor mediated reprogramming. ![]() ![]() Cross section through one turn of the cochlea shows organization in the organ of Corti as a mosaic of sensory hair cells (one row of inner hair cells and three rows of outer hair cells) interdigitated by various supporting cell populations labeled from left to right (Inner border/phalangeal, Pillar, Deiters’ and Hensen’s). ( A) Diagram of the mouse inner ear shows the vestibular system (green) and the cochlea of the auditory system (red). ![]()
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